Effect of Carbon/Nitrogen Ratio and Inorganic Phosphate on Colistin Production

概要

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The effect of culture conditions on antibiotic production (colistin) was studied with Bacillus polymyxa var. KY-7584 in Morido's fermentation medium (4% corn meal, 2% (NH_4)_2SO_4,0.2%KH_2PO_4,0.05%MgSO_4・7H_3O, 0.01%NaCl, 0.01% acetic acid and 1% CaCO_3). A simplified medium comprising four major components (corn meal, (NH_4)_2SO_4,KH_2PO_4,and CaCO_3) of Morido's medium [Morido, T. : Nippon Nogeikagaku Kaishi, 36,24 (1962)] gave the same productivity as Morido's medium. Further studies of culture conditions with the simplified medium revealed the ratio of carbon to nitrogen (C/N) and KH_2PO_4 concentration in the medium to be important factors in colistin production. The optimum KH_2PO_4 concentration in the medium was remarkably influence by the (C/N) value. B. polymyxa var. KY-7584 produced 35,800 u/ml of colistin in the optimum fermentation medium, which was about three times as much as in Morido's medium (13,300 u/ml).
公益社団法人日本生物工学会の論文
1982-12-25