Use of Fluorochrome-Labeled rRNA Targeted Oligonucleotide Probe and Tyramide Signal Amplification to Improve Sensitivity of Fluorescence in situ Hybridization(METHODS)
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概要
- 論文の詳細を見る
A tyramide signal amplification (TSA) system was used in combination with a conventional fluorochrome-labeled 16S rRNA oligonucleotide probe to increase the sensitivity of fluorescence in situ hybridization. TSA was performed after hybridization resulted in a low fluorescence signal intensity. In contrast to the horseradish peroxidase-tyramide signal amplification (HRP-TSA) system and biotin-tyramide signal amplification (biotin-TSA) system, no additional expensive probe labeling was required. A whole cell hybridization technique was used to compare the fluorescence signal obtained using a monolabeled probe with that obtained using the TSA system. The fluorescence signal of the probe obtained using the TSA system was much higher than that obtained using the monolabeled probe. The technique was successfully applied to the in situ detection of microbial communities in anaerobic sludge. It was demonstrated that TSA resulted in an increased in sensitivity, as the fluorescence signal intensity was much higher than that obtained using a conventional probe.
- 公益社団法人日本生物工学会の論文
- 2004-10-25
著者
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Tanticharoen Morakot
Biochemical Engineering And Pilot Plant Research And Development Unit National Center For Genetic En
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Tanticharoen Morakot
Biochemical Engineering And Pilot Plant Research And Development Unit National Center For Genetics E
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Cheevadhanarak Supapon
School Of Bioresources And Technology King Mongkut's University Of Technology Thonburi
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Cheevadhanarak Supapon
School Of Bioresources King Mongkut University Of Technology Thonburi
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JUPRAPUTTASRI WIWAT
School of Bioresources, King Mongkut University of Technology Thonburi
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CHAIPRASERT PAWINEE
School of Bioresources, King Mongkut University of Technology Thonburi
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TECHKARNJANARUK SOMKIET
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetics
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Chaiprasert Pawinee
School Of Bioresources King Mongkut University Of Technology Thonburi
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Jupraputtasri Wiwat
School Of Bioresources King Mongkut University Of Technology Thonburi
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Techkarnjanaruk Somkiet
Biochemical Engineering And Pilot Plant Research And Development Unit National Center For Genetics E
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TANTICHAROEN MORAKOT
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetics Engineering and Biotechnology, King Mongkut University of Technology
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CHEEVADHANARAK SUPAPON
School of Bioresources, King Mongkut University of Technology Thonburi
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- Use of Fluorochrome-Labeled rRNA Targeted Oligonucleotide Probe and Tyramide Signal Amplification to Improve Sensitivity of Fluorescence in situ Hybridization(METHODS)
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