Differential Responses of Three Acyl-Lipid Desaturases to Immediate Temperature Reduction Occurring in Two Lipid Membranes of Spirulina platensis Strain C1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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In cyanobacteria, the elevation of unsaturated fatty acid levels in membrane lipids has been shown to play a major role in the response to temperature change. The cyanobacterium Spirulina platemis strain C1 contains three desaturases - Δ^9, Δ^<12> and Δ^6 desaturases - which are encoded by desC, desA and desD, respectively. In light of the above, a study was conducted of the regulation of desaturase gene expression in response to temperature change in S. platensis strain C1. The two lipid membranes, thylakoid and plasma, were separated, while the expressions of the desaturase genes to the downward shift of growth temperature were studied in the translation level by Western blot analysis. The results revealed that the expression of Δ^9 desaturase is independent of temperature. In the case of Δ^<12> desaturase, two forms of the enzyme were found, at 45 and 40 kDa. In terms of correlation with the results in the transcription level, it is more likely that the 45kDa-Δ^<12> desaturase and the 40kDa-Δ^<12> desaturase are translated from 1.7kb and 1.5kb mRNA, respectively. Taken together, the results indicate that the expression of the 40 kDa-Δ^<12> desaturase is temperature independent, whereas, the 45 kDa protein form demonstrates a response to the immediate temperature reduction. Furthermore, the activity of Δ^6 desaturase in the two lipid membranes is possibly regulated by temperature reduction. However, alteration in the level of y-linolenic acid, the product synthesized by Δ^6 desaturase, was observed in the plasma membrane prior to the thylakoid membrane.
社団法人日本生物工学会の論文
2003-12-25

Differential Responses of Three Acyl-Lipid Desaturases to Immediate Temperature Reduction Occurring in Two Lipid Membranes of Spirulina platensis Strain C1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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