Light Regulation of Phycobilisome Structure and Gene Expression in Spirulina platensis C1(Arthrospira sp.PCC 9438)

スポンサーリンク

概要

• 論文の詳細を見る

• Light intensity is the most significant environmental factor influencing the light harvesting complexes (phycobilisomes) in cyanobacteria. Spirulina platensis C1, a filamentous cyanobacterium, was grown in batch culture under low (50 μE m^<-2>s^<-1>) and high (500 μE m^<-2>s^<-1>) photosynthetic photon flux density. Phycobilisomes were isolated, purified and analyzed by spectrophotometry, gel electrophoresis and electron microscopy. The absorption spectra in conjunction with the electron micrographs revealed that they are hemidiscoidal with three allophycocyanin cylindrical cores and six phycocyanin rods radiating from the core. The 33 kDa linker polypeptide normally found in low light cells disappeared under high light resulting in smaller phycobilisomes. The decrease in the number of phycobilisomes correlated well with the decrease in phycocyanin and allophycocyanin content from cells shifted from low to high light. The phycobiliprotein transcripts exhibited different pattern in response to different light intensities. Three species of phycocyanin mRNA of sizes 1.4, 1.5 and 3.5 kb and two species of allophycocyanin of 1.4 and 1.7 kb were found in low light grown cells. However in high light, the 3.5 kb phycocyanin transcript disappeared while the others remained with subtle changes. This observation indicated that the largest transcript encoding for phycocyanin subunits and linker polypeptides and the phycobilisome structure are modulated by light intensity.

著者

• Tanticharoen Morakot

  School Of Bioresources And Technology King Mongkut's University Of Technology Thonburi:national

• Cheevadhanarak Supapon

  School Of Bioresources And Technology King Mongkut's University Of Technology Thonburi

• Thomas Jean

  Laboratoire De Photoregulation Et Dynamique Des Membranes Vegetales Cnrs Ura 1810 Ecole Normale Supe

• De Marsac

  Unite De Physiologie Microbienne Institut Pasteur

• Nomsawai Pinthipya

  School of Bioresources and Technology, King Mongkut's University of Technology Thonburi

• Nomsawai Pinthipya

  School Of Bioresources And Technology King Mongkut's University Of Technology Thonburi:unite De

関連論文

• Identification of a heat shock-responsive cis-acting DNA sequence and its transcriptional regulator : Their roles in the expression of the Spirulina-desD gene in response to heat stress(GENETICS, MOLECULAR BIOLOGY, AND GENE EXPRESSION)
• Differential Responses of Three Acyl-Lipid Desaturases to Immediate Temperature Reduction Occurring in Two Lipid Membranes of Spirulina platensis Strain C1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Light Regulation of Phycobilisome Structure and Gene Expression in Spirulina platensis C1(Arthrospira sp.PCC 9438)
• Use of Fluorochrome-Labeled rRNA Targeted Oligonucleotide Probe and Tyramide Signal Amplification to Improve Sensitivity of Fluorescence in situ Hybridization(METHODS)

スポンサーリンク