Recent Studies of Protein Secretion by Filamentous Fungi(BREWING AND FOOD TECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• Filamentous fungi have been widely exploited for the homologous and heterologous protein production, because of the high capacity of their protein secretion machinery. However, the production of heterologous proteins is often limited while the production of homologous proteins can be very high. Various researches have reported the methods for overcoming this problem and some techniques, such as the fusion gene system, improve the production of heterologous proteins. Recently, the molecular biological study of solid-state culture attracts the attention, because the long history of biological studies has shown that the productivity of protein in the solid-state culture frequently exceeds the productivity of protein in the submerged culture. The recent researches of solid-state culture have revealed the new aspects of protein production in filamentous fungi. Solid-state specific gene expression was observed in the glaB and pepA genes of Aspergillus oryzae. A GC-box and HSE element of the glaB promoter region affected solid-state specific gene expression of this gene. Solid-state culture-specific release of enzymes from the cell wall was also observed in the production of β-glucosidases in Aspergillus kawachii. Extracellular soluble polysaccharide (ESP) from A. kawachii was concerned with the location of β-glucosidases. Moreover, ESP and the cell wall fraction of A. kawachii were shown to be involved in the stability of β-glucosidases. The knowledge of the molecular biology of solid-state culture should provide new approaches for the production of both homologous and heterologous proteins in both submerged culture and solid-state culture of filamentous fungi.

• 社団法人日本生物工学会の論文
• 2002-12-25

著者

• Ito K

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• IWASHITA Kazuhiro

  National Research Institute of Brewing

• Iwashita K

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

関連論文

• Effects of Culture Conditions on Ergosterol Biosynthesis by Saccharomyces cerevisiae
• Cloning and Analysis of the AWA1 Gene of a Nonfoaming Mutant of a Sake Yeast
• Amplified Fragment Length Polymorphism of the AWA1 Gene of Sake Yeasts for Identification of Sake Yeast Strains(BREWING AND FOOD TECHNOLOGY)
• Extracellular Soluble Polysaccharide (ESP) from Aspergillus kawachii Improves the Stability of Extracellular β-Glucosidases (EX-1 and EX-2) and Is Involved in Their Localization
• Production and Some Properties of Salt-Tolerant β-Xylosidases from a Shoyu Koji Mold, Aspergillus oryzae in Solid and Liquid Cultures
• Purification and Characterization of Extracellular and Cell Wall Bound β-Glucosidases from Aspergillus kawachii
• Recent Studies of Protein Secretion by Filamentous Fungi(BREWING AND FOOD TECHNOLOGY)
• Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Bitter-Tasting Sake Peptides Derived from the N-Terminus of the Rice Glutelin Acidic Subunit
• Cloning and Sequencing of the xynC Gene Encoding Acid Xylanase of Aspergillus kawachii

スポンサーリンク