ATTENUATION OF MYOCARDIAL STUNNING BY AN INCREASE IN THE H^+ BUFFERING CAPACITY OF THE PERFUSATE AND THAT BY HYPOXIC PREPERFUSION ARE AFFIECTED DIFFERENTLY BY THE FREE[Ca^<2+>]OF THE PERFUSATE
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概要
- 論文の詳細を見る
Objectives: Protons produced during ischemia may increase intracellular Na^+ (Na^+i) through Na^+/H+ exchange, and may lead to Ca^<2+> overload through Na^+/Ca^<2+> exchange to cause myocardial stunning. This study investigated whether an increase in the H^+ buffering capacity of the perfusate or a reduction of H^+ production by a brief hypoxic preperfusion before ischemia would reduce myocardial stunning. We also investigated whether the protective effect of these maneuvers depends on the free [Ca^<2+>] of the perfusate. Methods: Isolated rat hearts were preperfused with oxygenated or hypoxic buffer (pH 7.4) containing 100 mM of either sucrose or HEPES for 10 min, followed by 15 min of total ischemia and 30 min of reperfusion. To investigate the dependence of the effects of HEPES or a brief hypoxic preperfusion, the free Ca^<2+> concentration in the buffer was changed from 1.25 mM to 2.5 mM in some hearts. Results: Oxygenated preperfusion with buffer containing HEPES and 1.25 or 2.5 mM Ca^<2+> improved the metabolic and functional recovery with a decrease in the accumulation of Na^+ i during ischemia and in ^<45>Ca^<2+> uptake during re-perfusion. A brief hypoxic preperfusion with 1.25 mM Ca^<2+> provided a similar protective effect whereas no protective effect was observed when the [Ca^<2+>] was raised to 2.5 mM. Conclusions: An increase in the H+ buffering capacity or a brief hypoxic pre-perfusion reduced myocardial stunning with improved metabolic recovery, and reduced Ca^<2+> uptake. However, the effects of these interventions were affected differently by the free [Ca^<2+>] of the perfusate, which suggests that they work, at least in part, through some different mechanism(s).
- 社団法人日本循環器学会の論文
- 1993-11-20
著者
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Shinmura Ken
Department Of Geriatric Medicine Keio University School Of Medicine
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Tani Masato
Department Of Geriatric Medicine Keio University School Of Medicine
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Tani Masato
Cardiopulmonary Division Department Of Medicine School Of Medicine Keio University
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Asakura Yasushi
Department of Caridology, Toyohashi Heart Center
-
Asakura Yasushi
Department Of Cardiology Toyohashi Heart Center
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Asakura Yasushi
Cardiopulmonary Division Department Of Medicine Keio University School Of Medicine
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NAKAMURA Yoshiro
Department of Chemistry, Faculty of Science, Konan University
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Nakamura Yoshiro
Geriatrics, School of Medicine, Keio University
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Nakamura Y
Geriatrics School Of Medicine Keio University
-
Nishikawa Yasuhiro
Cardiopulmonary Division Dept. Of Med. School Of Med. Keio Univ.
-
Nakamura Yoshiro
Department Of Internal Medicine School Of Medicine Keio University
-
Ebihara Yoshinori
Departments of Geriatric Medicine, Keio University School of Medicine
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HANDA SHUN-NOSUKE
Department of Internal Medicine, School of Medicine Keio University
-
Nakamura Yoshiro
Dept. Of Intern. Med. Keio Univ. School Of Med.
-
Tani Masato
Department Of Cardiology
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Tani Masato
Cardio-pulmonary Division School Of Medicine Keio University
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Nakamura Yoshiro
Cardio-pulmonary Division Department Of Medicine Keio University School Of Medicine
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Nakamura Yoshiro
Cardiopulmonary Division Department Of Medicine School Of Medicine Keio University
-
Nakamura Yoshiro
Department Of Internal Medicine Keio University Hoipital
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HANDA Shunnosuke
Tokai University School of Medicine
-
Handa Shunnosuke
Department Of Cardiology School Of Medicine Tokai University
-
HANDA Shunnosuke
Health Evaluation and Promotion Center Tokai University Hospital
-
Nishikawa Yasuhiro
Department Of Internal Medicine School Of Medicine Keio University
-
Nakamura Yoshirou
Cardiopulmonary Division School Of Medicine Keio Univ.
-
Ebihara Yoshinori
Departments Of Geriatric Medicine Keio University School Of Medicine
-
Handa Shunnosuke
Department Of Internal Medicine School Of Medicine Keio University
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Nishikawa Yasuhiro
Cardiopulmonary Division Department Of Medicine Keio University School Of Medicine
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Nakamura Yoshiro
Department Of Chemistry Faculty Of Science Konan University
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Nakamura Yoshiro
Cardiopulmonary Division. Department Of Internal Medicine Keio University
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Nakamura Yoshirou
Cardiopulmonary Division. Department Of Internal Medicine Keio University
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Handa Shun-nosuke
Department Of Internal Medicine School Of Medicine Keio University
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EBIHARA Yoshinori
Department of Geriatric Medicine, Keio University School of Medicine
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