蛔虫アレルギーにおける抗原および抗体に関する研究 : 第11報 PCA反応による血中蛔虫抗体の証明

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With the purpose to trace the change of circulating antibody titer in ascaris allergy, Kuroume of this department previously applied the tanned cell agglutination technique which was, initiated by Boyden, and reported that an observable change in circulating antibody titer had been recognized not only in experimental but also in human ascariasis. Zoltan Ovary devised PCA (passive cutaneous anaphylaxis) using guinea pig skin, and found it positive for egg albumin and polysaccharide of Diplococcus Pneumoniae. This is a test of interest for antibodies, but nevertheless have not be applied in the field of parasitology. The author applied this test for ascariasis, and demonstrated circulating ascaris PCA antibody in ascaris-infected and ascaris body fluid-sensitized guinea pigs and also in animals bred in farmer's house without any precautions. And the author investigated relation among results of this test, tanned cell hemagglutination and precipitation. As the ascaris body fluid is used as the antigen intravenously in this animal experiment, for intravenous injection, it is of utmost necessity that guinea pigs must be non-infected, and to survive intravenous injection of a considerably large dose of the ascaris body fluid. On this account, the author used animals which were bred by special breeding method devised by this department in which animals are kept free from ascaris infection. Sera to be tested, either original or saline dilution, were intracutaneously injected in the abdomen in a dose of 0.1 ml., and after 4-6 hours, a mixture of each 0.5 ml of 10% body fluid and 0.5% Evans blue was intravenously injected. Other 15 minutes later, the site of the serum injection was examined, and when the diameter of blue staining was over 5 mm, the reaction was evaluated as positive. Results of the experiment in which the antibody absorbed completely by adding the same volume of the ascaris body fluid or the serum from non-infected guinea pig was used, was always negative. It is therefore, assumed that this reaction might be specific to ascaris. When non-infected guinea pigs were sensitized by single injection of 1 ml/body fluid, level of PCA antibody titer was proved to become to the highest after 3 weeks. In the experiment in which 5,000 ascaris ova were orally given at one time to non-infected guinea pigs, the level of the antibody became highest after 2 months. PCA antibody titer did not always change in parallel with titers of the hemagglutinin and precipitin, and therefore the former is considered to be different from the latter. Experiment for thermostability revealed that PCA antibody was destroyed by heating at 67℃ for 30 minutes. And out of 53 guinea pigs, which were bred without any precautions, 24 (45%) were positive in this reaction. From the results, it was thought that naturally bred guinea pigs in Japan might be infected with ascaris in variable grade.
日本アレルギー学会の論文
1961-07-30

著者

高橋良
群馬大学小児科学教室

蛔虫アレルギーにおける抗原および抗体に関する研究 : 第11報 PCA反応による血中蛔虫抗体の証明

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