10. 1) 血漿 Kallikreinogen の測定法について 2) Kinin の新しい Bioossay の方法について

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(1) Plasma kallikrein is ordinarily present in circulating plasma as an inactive precursor, kallikreinogen. In 1955, it was reported by Werle that a proteolytic enzyme, trypsin, was capable to convert plasma kallikreinogen to active kallikrein. An attempt for the assay method of kallikreinogen level in human plasma was made with the application of this property of trypsin. Two tenths milliter of human plasma was incubated with 200 meg of trypsin at 37℃ for 10 minutes, then 200 mcg of egg white trypsin inhibitor was added to the incubation mixture to inactivate the trypsin, and the produced kallikrein was estimated by its depressor effect on the rabbit blood pressure. Plasma kallikreinogen levels in healthy persons ranged from 3.5 to 4.4 Freu units per ml of plasma. Low kallikreinogen levels were observed in some patients with hyperthyroidism, and with hepatitis. In some patients with bronchial asthma and Buerger's disease, rather high levels wre found. (2) The autoperfused hind quarter preparation of the rabbit used for the assay of kinin. Perfusion of the hind quarter was performed at a conastant flow rate by a Sigma motor pump interposed between the carotid and the femoral artery. The femoral artery of the rabbit was very sensitive to kinin and the intraarterial injection of 0.5 ng of synthetic bradykinin produced a detectable vasodilatation in most animals. This organ was l0-20 times more sensitive to this polypeptide than a dog hind quarter. Dose-depending responses were obtained. Hisamine and serotonin produced the pressor responses in 1000 times lager quantity than that of synthetic bradykinin on this organ. On the other hand, depressor responses were observed by acetylcholine and adenosine triphosphate. Acordingly, a contamination of these substances should be completely avoided or, if this could not be achieved, the action of them should be blocked by specific inhibitors when the activity of kinin are determined by the autoperfused hind quarter of the rabbit.
日本アレルギー学会の論文
1969-06-30

10. 1) 血漿 Kallikreinogen の測定法について 2) Kinin の新しい Bioossay の方法について

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