Photorepair and Excision Repair Removal of UV-induced Pyrimidine Dimers and (6-4) Photoproducts in the Tail Fin of the Medaka, Oryzias latipes

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Induction and repair of UV-B induced DNA damage in the tail fin of the Medaka, were examined immunohistochemicaly and by the enzyme-linked immunosorbent assay (ELISA). UV-induced DNA damage was detected only in the outermost layer of epithelial cells and did not differ in fishes having different degree of melanization. Both pyrimidine dimers and (6-4) photoproducts in the fin cells were removed by excision repair in the dark, the excision of (6-4) photoproducts being about twice as efficient as that of pyrimidine dimers. The rate of excision repair of UV-induced lesions in fin tissue was three to four times that in cultured Medaka cells, OL32. . In the fin cells, reductions in the numbers of pyrimidine dimers and (6-4) photoproducts were seen after treatment with fluorescent light, whereas less reductions of pyrimidine dimers and no reductions of (6-4) photoproducts were observed in OL32 cells. There are two major types of UV-induced DNA damage: primidine dimers and (6-4) photoproducts.
日本放射線影響学会の論文