Adenine-N-Oxide Produced from Adenine with Gamma-Rays and Its Binding to SH Protein

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^<14>C-labeled adenine aqueous solution was irradiated with ^<60>Co gamma-rays. The yield of adenine-7-N-oxide, a radiolytic product, was determined by Sephadex G-10 column chromatography and TLC autoradiography. The apparent productive yield was very low, but the true yield should be much higher because of the reversible reaction to adenine and the easy decomposition of the N-oxide itself. Using synthesized ^<14>C-adenine-7-N-oxide, noncovalent binding of this N-oxide to urease, an SH protein, was confirmed in comparison between the presence and absence of SDS by Ultrogel AcA 22 column chromatography. The noncovalent binding of the gamma-irradiated ^<35>S-cysteine was also observed. The yield reached a limit in O_2 easier than in N_2 as the atmosphere for DNA irradiation. These results support an interaction structure, [chemical formula], for noncovalent binding which may be applied to the biological system as a radiation-induced damage.
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