Radiation-Induced Binding of 4-Nitroquinoline-N-Oxide to DNA in Aqueous Solution

概要

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4NQO-DNA mixture solution was irradiated with ^<60>Co gamma-rays. Binding yield of 4NQO to DNA was compared to that of cysteine to DNA after fractionation by gel filtration. Their binding yields increased with increase in radiation dose. The dose vs binding yield curves of cysteine to single stranded DNA, cysteine to double stranded DNA, and 4NQO to double stranded DNA showed first order kinetics, but that of 4NQO to single stranded DNA was found to be different from the others i. e, it showed that the reaction was a second power function of radiation dose. 4NQO (4-nitroquinoline-N-oxide), a chemical carcinogen, is structually aromatic and has a characteristic NO group. The presence of noncovalent crosslinking in vitro between 4NQO and DNA without radiation has been reported. A study has been made by Tada et al. on the binding of 4NQO to DNA promoted with UV (personal communication). However, no data is available on 4NQO-DNA binding caused by ionizing radiation. In this paper, the radiation-induced binding activity of 4NQO to DNA is compared to that of cysteine which is a high radiation-induced binding participant.
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