C57BL/6Jマウス可移植性悪性グリオーマの脳内移植に関する免疫組織学的研究

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Transplantable gliomas of C57BL/6J mice were used in order to investigate the influence of immunological resistance against tumors transplanted in the right parietal lobe. Immunization was induced by subcutaneous inoculation of approximately 1 mm^3 of tumor grafts, irradiated by 3,000 watt.sec/cm^2 ultrasound or 3,000 rad X-ray, once a week for 2 weeks to normal animals. Immunization was assured by tumor rejection or no growth 2 to 3 weeks after challenge of non-treated tumor implants. The rates of immunization against tumor were 67% by ultrasound irradiation and 45% by X-ray irradiation.Passrve transfer Of resustance against the turnor was evaluated by IV injection of 1 X 10^8 spleen cells frorn immunized animals to normal isologous animals. The result showed a 40% rate of transfer. The rate of tumor growth transplanted in the brain was 71% in immunized mice induced by subcutaneous inoculation with no significant difference from normal mice (81%). However,survival intervals after transplantation were significantly longer (P< 0.01), ranging from 17.6 to 23.7 days. The tumor was more localized and showed more degeneration.Lymphocytic infiltration into the brain tumor was much less pronounced than subcutaneous tumor in immunized animals and there was no marked glial reaction. Focal lymphocytic accumulations were sometimes observed in perivascular spaces and in the meninges, but there were no clear differences from non-immunized tumor-bearing mice. The population of T-cells in the spleen and the mesenteric lymphnodes was evaluated by acid α-naphthyl acetate esterase (ANAE) stain in imprint preparations and in freshly snap-frozen (-80℃) and cryostat-cut specimens of the spleen, lymphnodes and brain tumors. ANAE stain reported by Mueller in 1975 demonstrated one or two positive dots in T-cells. T-cells in the spleen and lymphnodes increased but only a few were detected in the brain tumor of the immunized animals. Direct immunofluorescent studies with FITC-labelled anti-mouse Ig antibodies were applied to freshly snap-frozen and cryostatcut specimens. As a result, Ig-cotaining cells infiltrated the tissues around the subcutaneous tumor while a few penetrated the tumor tissues. Fluorescence was not marked on the cytoplasmic membrane of brain tumor of immunized animals but was positive in those of non-immunized animals and was more marked in those animals which showed tumor growth in spite of induction of immunization, suggesting that the blocking factor described by Hellstrom was involved.
日本脳神経外科学会の論文
1978-08-15

C57BL/6Jマウス可移植性悪性グリオーマの脳内移植に関する免疫組織学的研究

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