胆道閉鎖症の肝組織における炎症性細胞浸潤の動態 : モノクローナル抗体による免疫組織化学的検討

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Infiltrating pattern of inflammatory cells and expression of gamma-glutamyltransferase (GGT) were examined by immunohistological and histochemical techniques in the liver of biliary atresia (BA). Twenty four specimens were obtained from 20 case of BA at the time of the radical, re-boring operation or conversion of enterostomy. The monoclonal antibodies used in these studies were CD4, CD8, CD11, CD15, CD20, antibodies to IL-1 and HLA D/DR. GGT was stained by Rutenberg's method. The area of GGT positively stained and numbers of infilatrating cells were surveyed by the immage analizer. The inflammatory cells were composed of CD4^+ (helper/inducer T) cells, CD8^+ CD11^- (cytotoxic T) cells and CD15^+ (granulo/mono) cells. The infiltrating pattern at the place of canaliculo-ductal junction (C-Dj.) was characterized by collection of CD8^+ CD11^- (cytotoxic T) cells and CD4^+ (helper/inducer T) cells. At those C-Dj., it was observed that hepatocytes and bile duct epithelial cells were progressively damaged and fell off. The cellular damage at C-Dj. led proliferation of bile duct, where GGT and deposition of bile pigments were induced intensively. Once the inflammation of C-Dj. spread into the hepatic lobule, the bile pigment and GGT, which cannot be seen normally, were observed at the bile canaiculi, because of the obstruction of bile transporting system. The number of infiltrating cells, the area of GGT and depositing bile pigment were closely related with serum total bililubin, serum GGT and other laboratory data.
日本小児外科学会の論文
1989-04-20

胆道閉鎖症の肝組織における炎症性細胞浸潤の動態 : モノクローナル抗体による免疫組織化学的検討

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