身体運動後の尿タンパク質の未変性 : 2次元電気泳動法による分析

概要

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A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19〜21 years) were loaded physical exercise with a 10 km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68〜2.56 mg/dl), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in a addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.
日本体力医学会の論文
1993-04-01