41. タバコ N 因子によるタバコモザイクウィルスの認識機構の解析

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Plant resist invading microbial pathogens by mounting rapid defense responses. For instance, tobacco plants carrying the N gene show a hypersensitive response (HR) against tobacco mosaic virus (TMV). This response can act only when the plant has a resistance gene and the pathogen has an avirulence gene. In the case of tobacco and TMV, N is a resistance gene product and the 50 kDa TMV helicase domain (p50) is an avirulence gene product. However, it is not yet known how the N factor recognizes p50. We investigated the interaction of the N protein and p50 by the yeast Two-hybrid system. When both the N protein and p50 are present, β-galactosidase activity is about 10 times higher as compared to the presence of only one of the two. Next, the specificity of the interaction was investigated by the growth test using a histidine synthase inhibitor (3-AT). Our results shown that, only when both the N factor and p50,but not one of the two alone, are present growth was possible. Several strains of TMV have been reported not to induce the HR in tobacco. One of these strains has an amino acid exchange in the hericase domain. A possible explanation of the no-HR phenotype would be a disturbed interaction with the N protein. For this reason we checked this mutated p50 in the yeast Two-hybrid assay. In contrast to the WT protein, the mutated p50 resulted in a very lowβ-galactosidase activity. Moreover, it did not grow in the 3-AT containing culture medium. The results suggest that p50 has combined with N factor directly
植物化学調節学会の論文
2003-10-10

41. タバコ N 因子によるタバコモザイクウィルスの認識機構の解析

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