D-40 ジベレリン非感受性トウモロコシ突然変異株D8でmRNA蓄積量の低いcDNAの単離

概要

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A sigle gene dwarf mutant of maize, D8 is insensitive to exogeneously applied GA, despite its high level of endogeneous GA. This suggeste that D8 has defects either in receptor(s) or in signal transduction pathways. In order to isolate genes that are affected in the D8 mutant, we have screened and compared mRNA population between wild-type and D8 shoots by differential display. Subsequently, one cDNA clone, of which mRNA level was lower in D8 than in wild-type plants, was isolated and designated as gar2. The gar2 cDNA sequence contained a 900 base open reading frame capable of encoding a polypeptide of 177 amino acid residues. DNA blot analysis indicated two to three copies for gar2 in maize genome. RNA blot analysis showed that mRNA of gar2 was increased in response to exogeneously applied GA in maize GA-deficient mutant, d5, and that gar2 mRNA was abundant in leaf sheath containing meristematic tissues in wild-type plants.
植物化学調節学会の論文
1998-10-01