2. ジベレリンによって制御されるアポプラストタンパクの SDS-PAGE による検出

概要

論文の詳細を見る
In order to identify the gibberellin (GA_3)-regulated protein factors to regulate the mechanical properties of cell walls, we analyzed cell-wall proteins by SDS-PAGE extracted from pea (Pisum sativum L. cv. Alaska) roots, which had been treated with or without GA_3 and/or Ancymidol. GA_3-affected protein bands were separated, N-terminal amino acids were sequenced, and their homologies were surveyed on data bases. Eight protein bands were sequenced among 11 bands separated. Homologous proteins found on data base were as follows : GA_3-enhanced bands : pectin methylesterase (Pisum sativum, Vigna radiata), peroxidase (Spinacia oleracea, Glicine max, Arabidopsis thaliana) GA_3-suppressed bands : chitinase class III; (Vigna angularis, Nicotiana tabacum, Oriza sativa), cystein proteinase (Pisum sativum, Arabidopsis thaliana), peroxidase (Spinacia oleracea, Medicago truncatula). GA_3-enhanced methylesterase and peroxidase were suggested to participate in cell wall modification for GA-induced growth. GA_3 was suggested to regulate the amount of these apoplastic proteins. Further identification of unsequenced protein bands is in progress.
植物化学調節学会の論文
2003-10-10