ブラシノステロイド関連性遺伝子に関する分子生物学的研究

概要

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There has been limited information how BR biosynthesis is regulated and where BRs are synthesized and used. To address this issue, we isolated two BR-6-oxidase genes (BR6ox) from Arabidopsis and analyzed their gene expression with distribution of endogenous BRs. The results suggested that BRs are synthesized in all of the organs tested, but most actively synthesized in young actively developing organs. To further gain insight into the site of BR biosynthesis, transgenic Arabidopsis carrying reporter, BR6ox-GUS gene were produced. Using these lines, the site of biosynthesis and action is now under investigation at the cellular level. Although recent studies of biosynthesis and sensitivity mutants have provided insights into the functions of BRs, the mode of action of BRs is poorly understood. Using microarray analysis, we identified BR-regulated genes. The list of BR-regulated genes includes transcription factor genes, auxin-related genes, P450 genes, and genes implicated in cell expansion and cell wall organization. These results provide a comprehensive view of the actions of BRs, using BR-regulated genes as molecular markers. Despite numerous physiological studies addressing the interactions between BRs and auxin, little is known about the underlying molecular mechanisms. Using auxin-reporter gene system and auxin-related mutants, signaling interaction between auxin and BR have been investigated.
植物化学調節学会の論文
2003-10-10