Ampicillin耐性Staphylococcus aureusの酵素活性メカニズムと耐性菌中に存在するPlasmid DNA

概要

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We investigated the enzymatic mechanisms of resistance to antibiotics and the profiles of plasmid DNA in ampicillin-resitant Staphylococcus aureus (10 strains) isolated from clinical materials. _β-Lactamase activity was determined using the acidmetric disk method; the PCase (penicillinase) activity was observed in all except one strain tested, whereas no CSase (cephalo sporinase) activity was noted. All of the strains tested were susceptible in vitro to AMK, MINO and VAN, but were resistant to _β-lactam atibiotics. Antibacterial activity of AMK against all of the strains tested was less than 6.25μg/ml (MIC) and that of GM was more than 12.5μg/ml. Compared to the profiles of plasmid DNA derived by the electrophoresis pattern, the strains tested can be classified into two groups: Group I (6 strains) produced two plasmid DNA patterns obtained from plasmid DNA larger than the 23 kbase and around the 564 base, and Group II (4 strains) produced a different patter to those of Group I. These findings indicate that DNA plasmid larger than 23 dbase in ARSA is thought to be affect the resistance against _β-lactam and aminoglycoside antibiotics, such as through modification of the chemical structure.
日本医療薬学会の論文
1993-10-20