ジゴキシン血中濃度測定におけるラジオイムノアッセイとホモジニアスエンザイムイムノアッセイとの比較

概要

論文の詳細を見る
Serum of 43 Patients under digoxin therapy and 6 ampoules of high concentration digoxin-spiked serum were analyzed by homogeneous enzyme immunoassay (EMIT) and ^<125>I-radio-immunoassay (^<125>I-RIA). The correlation coefficient was 0.923 and the regression line was Y=1,235X-0.195 (Y:EMIT, X:^<125>I-RIA). Hourly change in the EMIT digoxin assay was determined by 2 specimens of control serum containing 1.0 and 3.0ng/ml of digoxin, and 3 specimens of mixed serum of patients. The coefficient of variation (CV) was less than 5.1%. Daily change in the assay was determined by 5 calibrators for 7 months and a control serum containing 1.0ng/ml of digoxin for 5 weeks. The CV was less than 7.1%. EMIT digoxin assay was thus recognized as a good substitute for RIA method ; especially it is recommended in hospital laboratories.
日本医療薬学会の論文
1981-12-20