イネカルス由来幼苗における葉の異常とその発達

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Gross morphological strudies have been conducted on the leaves that mainly developed from the shoot buds originated from rice callus cultures. Besides this, various leaf-like structures emerged from the callus prior to the appearance of shoot buds. These leaf-like structures were either attached to the basal part of shoot buds or were found in their vicinity. These observation lead to the presumption that shoot apices that give rise to shoot buds, in rice callus develop near these leaf-like structures. Also these shoot apices later have the ability to form leaf primordia which ultimately develop into leaves showing different morphologies. Interestingly, the leaves that appeared earlier showed abnormal features such as having multiple tips, showing the absence of ligule or abnormal ligules. As the growth of shoot bud matured, mormal leaves formed. Hence in a mature plantlets, abnormal leaves were seen at the base whereas normal ones were found near the shoot apex. These normal leaves resembled the leaves of rice seedling. Therefore, the shoot apices that formed in rice callus showed a tendency to form normal leaves at a later stage of development. It can also be inferred that besides shoot apex there are other factor/s also which help in the formation of normal leaves, because intimate relationship, between ligule formation and root formation in plantlets originated from rice callus, has also been observed. On the basis of the results so obtained and the earlier reports, we have chiefly described the gross morphological characteristics of leaves that developed from rice callus cultures.
日本作物学会の論文
1981-03-31

イネカルス由来幼苗における葉の異常とその発達

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