シリアン・ハムスター胎仔(SHE)細胞による非変異原性がん原物質のがん化機構の解析

概要

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The mechanisms of carcinogenesis induced by putative non-mutagenic carcinogens were studied using Syrian hamster embryo(SHE)cells. SHE cells were treated with 33 chemicals, which are negative in Salmonella mutagenicity tests, and assayed for determining the abilities of the chemicals to induce cell transformation and genetic effects. Twenty-two of the 33 chemicals are reported to be carcinogenic in rodents or humans. Treatment of SHE cells with each of the carcinogenic chemicals, except for clofibrate and progesterone, induced morphological transformation of the cells. Fifteen of 20 chemicals, which were positive for SHE cell-transforming activity, were positive in one or more genotoxicity tests including gene mutations at the hprt and/or Na^+/K^+ ATPase loci, chromosomal mutations(structural and numerical abnormalities), DNA damage detected by unscheduled DNA synthesis, and DNA adduct formation determined by ^<32>P-postlabeling. These findings indicate that the SHE cell assay system could be useful for mechanistic studies as well as for detecting potential carcinogenic activity of non-mutagenic carcinogens, and that genetic mechanisms may be important in the carcinogenic activity of non-mutagenic carcinogens.
日本環境変異原学会の論文
1998-07-21