<ORIGINAL ARTICLES>Electron Microscopic Examination of HRP-Labeled Corticogeniculo Neurons and Geniculocortical Afferent Terminals of Rats with Methylazoxymethanol-Induced Microcephaly

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Microcephaly which was induced by prenatal exposure to methylazoxymethanol (MAM) in rats (MAM-rats) shows such developmental anomalies as abnormal distribution and reduced number of corticogeniculate neurons and abnormally dense aggregation of geniculocortical afferent terminals. In the present study, horseradish peroxidase(HRP) was injected into the center of the dorsal lateral geniculate nucleus. The synaptic organization of the cortical neurons in layer VI and the HRP-labeled terminals of geniculocortical afferents in layer IV were the examined by and electron microscopi. No apparent abnormalities were observed in the synapses made btween the HRP-labeled perikarya of cortical neurons in layer VI and the HRP-negative terminals. However, the HRP-labeled terminals in layer IV formed different types of synapes between MAM-rats and controls. In the controls,HRP-labeled terminals sometimes formed symmetrical synapses with HRP-negative terminals and contained spherical vesicles, whereas asymmetrical synapses were found with HRP-negative terminals and contained pleomorphic vesicles in the MAM-rats. These findings attempt to give morphological understanding of the hebavioral and cognitive abnormalities observed in MAM-rats.
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