Application of Flow Cytometry for Measuring Changes in Intracellular RNA Contents of Marine Bacteria

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概要

• 論文の詳細を見る

• A flow cytometer was used to measure the intracellular RNA contents of Vibrio alginolyticus and natural bacterial populations after specific staining with acridine orange. During the course of starvation, the RNA level in Vibrio alginolyticus gradually decreased, whereas subsequent enrichment caused a rapid increment of RNA and resulted in the appearance of a distinct group on the cytometric histogram. Similar distinct subpopulations were also confirmed in natural seawater samples after 6 hours direct viable count (DVC) incubation. Moreover, it was confirmed that the DVC technique enabled us to detect and enumerate marine bacteria using 16S rRNA fluorescent oligonucleotide probes.

• 日本微生物生態学会の論文
• 1995-10-31

著者

• 大和田 紘一

  東大海洋研

• TSUDA Atsushi

  Hokkaido National Fisheries Research Institute

• Kogure Kazuhiro

  ORI, The Univ. Tokyo

• Nishimura Masahiko

  ORI, The Univ. Tokyo

• Tsuda A

  Univ. Tokyo Tokyo Jpn

• Tsuda Atsushi

  Planktology Division Ocean Research Institute University Of Tokyo

• Ohwada Kouichi

  Prefectural Univ. Kumamoto Kumamoto Jpn

• Ohwada Kouichi

  Marine Microbiology Division Ocean Research Institute University Of Tokyo

• Ohwada Kouichi

  Ocean Research Institute University Of Tokyo

• Nishimura Masahiko

  Ocean Research Institute The University Of Tokyo

• Nishimura Masahiko

  Ocean Research Institute University Of Tokyo

• Tsuda A

  Hokkaido National Fisheries Research Institute

• Kogure Kazuhiro

  Ori The Univ. Of Tokyo Faculty Of Fisheries

• Kogure Kazuhiro

  Atmosphere And Ocean Research Inst. Univ. Tokyo

• Kogure Kazuhiro

  Marine Microbiology Laboratory Department Of Marine Ecosystems Dynamics Ocean Research Institute Uni

• Tsuda Atsushi

  Atmosphere And Ocean Research Institute University Of Tokyo

• NISHIMURA MASAHIKO

  Marine Microbiology Division, Ocean Research Institute, The University of Tokyo

• Kogure Kazuhiro

  Marine Microbiology Lab. Marine Ecosystem Dynamics Ocean Res. Inst. The Univ. Of Tokyo

• Ohwada Koichi

  Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto

• Kogure Kazuhiro

  Marine Microbiology Division Ocean Research Institute University Of Tokyo

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