高分解能・高速度CCDビデオマイクロスコープ : 拍動心における微小循環の観測・解析

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We developed a high resolution and high speed CCD intravital videomicroscope. The microscope system consists of a needle probe, camera body containing a CCD camera that has a high definition CCD image sensor and a high speed CCD sensor. The temporal resolution is 33 or 5 ms and the spatial resolution is 2.5 or 3.9 μm. We could clearly, continuously observe diameters of both subepicardial and subendocardial arterioles and venules throughout a cardiac cycle in a beating dog heart. The vascular compression by cardiac contraction decreased the diameters of both subendocardial arterioles and venules by about 20% , whereas subepicardial arterial diameter changed very little during the cardiac cycle and subepicardial venules increased in diameter during systole. The blood flow in the microvessels were successfully visualized by the high speed system with a bright microsphere. In conclusion, a newly developed microscope system is a powerful tool for observation of dynamic diameter and flow changes in coronary microvessels in a beating dog heart.
1996-01-25