運動前後におけるMyoglobinの変動

概要

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The anti-human Myoglobin anti-body, was obtained by immunization of Myoglobin purified from human skeltal muscle against the rabbit, was purified by the affinity chromatography using the CNBr-activated Sepharose 4 B and Sephadex G-100. Detactable sensitivity of the Myoglobin value was about 500 Pg/ml by the reversed passive hemagglutination (RPHA). Results of serum Myoglobin value change before and after the exercise are mentioned below. Mean value of serum Mb in the normal group (176 subjects), was measured by the 2^n dilution method, was 5.1±1.03, and that in male and female were 5.3±0.93 and 4.8±1.03 respectively, and so that in male showed higher. The value before the exercise (at rest) in athletes (38 subjects) was 5.9±0.77, and showed the significant difference as compared to that in non-athletes. The Mb values increased rapidly immediately after the exercise in athletes and non-athletes, and that showed a decreasing tendency successively after the exercise in athletes, but increased in non-athletes. The Mb values in urine before, immediately after and successively after the exercise were positive in 10, 5 and 12 subjects in athletes (38 subjects) respectively, And the values before and immediately after the exercise were negative in non-athletes, but that successively after the exercise was possitive in 4 of 5 subjects in non-athletes.
東京工芸大学の論文