エビガラスズメの実験昆虫化 : 蛹の休眠特性と長期保存

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The roles of photoperiod and temperature in pupal diapause induction and the effect of cold storage on diapause pupae in the sweet potato hornworm, Agrius convolvuli, were investigated. Diapause was induced in almost all insect under photoperiods ranging from 4L-20D to 12L-12D, and day length of 16h or more prevented diapause at 23℃. The critical daylength (50% induced) at 23℃ was 13.4h. Temperatures of 23 or 24℃ were most favorable for diapause induction under 12L-12D photoperiod. Diapause induction was suppressed strongly by lower (17℃) and higher (27℃) temperatures. These responses to photoperiod and temperature with respect to diapause induction were similar to those found in the California strain of the tobacco hornworm, Manduca sexta. The sensitivity of diapause induction to photoperiod and temperature extended to almost all larval stages, and the maximum sensitivity to prevent diapause was in the feeding period of the 5th instar. Diapause pupae kept at 25℃ emerged sporadically from 30 to 200 days after pupation, indicating that low temperature is not necessary to break diapause, However, cold treatment at 10℃ for 1 month shortened the duration of pupal period, and the treated pupae emerged within 21 days at 27℃. Diapause pupae could be kept at 10℃ for 8 months, and they emerged normally and laid an average of 271 eggs. Cold storage of diapause pupae could be useful for maintenance of laboratory colonies.
日本応用動物昆虫学会の論文
1995-02-25

エビガラスズメの実験昆虫化 : 蛹の休眠特性と長期保存

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