The establishment of a Colorado potato beetle (Coleoptera : Chrysomelidae) pupal cell line
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概要
- 論文の詳細を見る
A cell line (BCIRL-Lepd-SL1) was established from pupal tissue of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). The cell line has been in culture for three years in EX-Cell 401^<TM> medium supplemented with 10% fetal bovine serum, during which time it has undergone over 100 subcultures. The cell population is heterogenous in nature, consisting of round cells, trapezoid-like cells and elongated cells. The cell population doubling times at 28℃ were estimated to be 3.4 days at passage 56 and 2.3 days at passage 111. The cell line did not support replication of Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants carrying the genes for green or blue fluorescent proteins. The cell line was confirmed to have originated from the Colorado potato beetle by the DAF-PCR technique.
- 日本応用動物昆虫学会の論文
- 2002-08-25
著者
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Grasela James
Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea
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Goodman Cynthia
Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea
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Mcintosh A
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Goodman C
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Grasela J.
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Long Steve
Biological Control of Insects Research Laboratory, USDA, Agricultural Research Service
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McIntosh Arthur
Biological Control of Insects Research Laboratory, USDA, Agricultural Research Service
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Long Steve
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Mcintosh Arthur
Biological Control Of Insects Research Laboratory Agricultural Research Service U.s. Department Of A
関連論文
- シングルヌクレオキャプシド(HzSNPV)およびマルチヌクレオキャプシド(AcMNPV)バキュロウイルスの野生株と組換え体に対するタバコガ幼虫の感受性
- Sensitivity of heliothine larvae to wild-type and recombinant strains of a single nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus
- Comparison of in vivo activity of virus from a deleted polyhedrin-gene strain of a single-nucleocapsid baculovirus and a multiple-nucleocapsid baculovirus
- The establishment of a Colorado potato beetle (Coleoptera : Chrysomelidae) pupal cell line
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- Effects of viral rate and feeding time on mortality of early-, mid-, and late-stadium larvae of Helicoverpa zea (Lepidoptera:Noctuidae) fed Baculovirus heliothis
- Virus Production and Plaque-Forming Ability of Helicoverpa zea (Lepidoptera: Noctuidae) Clonal Cell Lines
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