Growth of a clonal cell line of Helicoverpa zea (Lepidoptera:Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV
スポンサーリンク
概要
- 論文の詳細を見る
A clonal cell line (BCIRL-HZ-AM1-11) of Helicoverpa zea was grown in stationary and suspension cultures in Ex-Cell 401^<TM> medium containing 10% fetal bovine serum at 28℃. The cell population doubling time was 22h in stationary culture as compared with 27h in suspension culture. A lag time of approximately 24h was observed during the first 24h of the suspension culture following initiation but no lag time was observed in the stationary culture. Maximum viral titers were achieved in stationary and suspension cultures at 120h (1.80×10^6 TCID_<50>/ml) and 168h (1.48×10^7 TCID_<50>/ml), respectively, following inoculation with the Helicoverpa zea baculovirus (HzSNPV/Br-CL2). Infected cells harvested at 168h from the 50ml suspension culture produced a total number of occlusion bodies of 3×10^9.
- 日本応用動物昆虫学会の論文
- 2001-08-25
著者
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Ignoffo Carlo
Biological Control Of Insects Research Laboratory U.s.department Of Agriculture Agricultural Researc
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Ignoffo C
U.s. Dep. Agriculture Agricultural Res. Serv. Mo Usa
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Ignoffo Carlo
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory
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McIntosh Arthur
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory
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Mcintosh A
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Goodman C
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Grasela J.
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Grasela James
USDA, USA
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Goodman Cynthia
USDA, USA
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Grasela James
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory
関連論文
- シングルヌクレオキャプシド(HzSNPV)およびマルチヌクレオキャプシド(AcMNPV)バキュロウイルスの野生株と組換え体に対するタバコガ幼虫の感受性
- Sensitivity of heliothine larvae to wild-type and recombinant strains of a single nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus
- バキュロウイルスの野生株または組換体を接種したHeliothis virescensおよびHelicoverpa(Heliothis)zea幼虫における封入体の産生量
- Mortality and feeding of mid-stadium larvae of Helicoverpa zea and Heliothis virescens fed a wild strain or a recombinant strain of Baculovirus heliothis expressing an insect-specific toxin of the scorpion Leiurus quinquestriatus hebraeus
- Comparison of in vivo activity of virus from a deleted polyhedrin-gene strain of a single-nucleocapsid baculovirus and a multiple-nucleocapsid baculovirus
- Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses
- The establishment of a Colorado potato beetle (Coleoptera : Chrysomelidae) pupal cell line
- 浮遊培養条件下でのタバコガHelicoverpa zeaクローン細胞系の増殖とバキュロウイルスHzSNPVの複製
- Growth of a clonal cell line of Helicoverpa zea (Lepidoptera:Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV
- Baculovirus heliothisの接種量と摂食時間がHelicoverpa zea(鱗翅目:ヤガ科)初齢, 中齢, および終齢幼虫の死亡率に及ぼす影響
- Effects of viral rate and feeding time on mortality of early-, mid-, and late-stadium larvae of Helicoverpa zea (Lepidoptera:Noctuidae) fed Baculovirus heliothis
- Yields of occlusion bodies from Heliothis virescens(Lepidoptera:Noctuidae)and Helicoverpa(Heliothis) zea(Lepidoptera:Noctuidae)larvae fed wild or recombinant strains of baculoviruses
- Virus Production and Plaque-Forming Ability of Helicoverpa zea (Lepidoptera: Noctuidae) Clonal Cell Lines
- Per os and intrahemocoelic activity of exocellular and intracellular virus from a wild-type and deleted polyhedrin-gene strain of the nucleopolyhedrovirus of Autographa californica