Comparison of in vivo activity of virus from a deleted polyhedrin-gene strain of a single-nucleocapsid baculovirus and a multiple-nucleocapsid baculovirus
スポンサーリンク
概要
- 論文の詳細を見る
Larvae of Trichoplusia ni, Helicoverpa zea and Heliothis virescens were assayed for their relative suscepti-bility to in vitro produced, nonoccluded virus (P^-NOV) from a deleted polyhedrin-gene strain of the nucleopolyhedroviruses of Autographa californica (P^-AcMNPV) and Helicoverpa zea (P^-HzSNPV). There was a 90-fold difference in P^- NOV activity (LC_<50> values based upon TCID_<50>/mm^2) between P^-AcMNPV (vs. T. ni) and P^-HzSNPV (vs. H. zea) when each virus was assayed against its respective permissive host. These differences in P^-NOV activity were within the same range one would expect based upon their reported LC_<50> values and the estimated numbers of virions embedded in occlusion bodies (OB) of the wild-type (Wt) isolate of these viruses. Differences in LC_<50> values (TCID_<50>/mm^2) between P^-NOV of P^-AcMNPV and P^-NOV of P^-HzSNPV using H. virescens larvae (a permissive host susceptible to both viruses) however, were much greater ( > 300-fold) than expected if based upon the number of virions/WtOB. Closer agreements in LC_<50> values between P^-NOV and occluded viruses of WtOB were obtained for H. zea or H. virescens larvae exposed to P^-HzSNPV (ca. 1.3-fold difference) or H, zea and H. virescens larvae exposed to P^-AcMNPV (ca. a 15-fold difference). Results using a non-susceptible host (P^-NOV of P^-HzSNPV vs. T. ni) or a semi-permissive host (P^-NOV of P^-AcMNPV vs. H, zea) generally agreed with previous citations using occluded virions of WtOB.
- 日本応用動物昆虫学会の論文
- 1999-02-25
著者
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Ignoffo Carlo
Biological Control Of Insects Research Laboratory U.s.department Of Agriculture Agricultural Researc
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Ignoffo C
U.s. Dep. Agriculture Agricultural Res. Serv. Mo Usa
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Ignoffo Carlo
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory
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McIntosh Arthur
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory
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Wong James
E. I. duPont de Nemours Co., Stine-Haskell Research Center, Agricultural Products
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Wong J
E.i.dupont De Nemours Co. Stine-haskell Research Center Agricultural Products
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Mcintosh A
Biological Control Of Insects Research Laboratory Usda Agricultural Research Service
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Wong James
E. I. Dupont De Nemours Co. Stine-haskell Research Center Agricultural Products
関連論文
- シングルヌクレオキャプシド(HzSNPV)およびマルチヌクレオキャプシド(AcMNPV)バキュロウイルスの野生株と組換え体に対するタバコガ幼虫の感受性
- Sensitivity of heliothine larvae to wild-type and recombinant strains of a single nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus
- バキュロウイルスの野生株または組換体を接種したHeliothis virescensおよびHelicoverpa(Heliothis)zea幼虫における封入体の産生量
- Mortality and feeding of mid-stadium larvae of Helicoverpa zea and Heliothis virescens fed a wild strain or a recombinant strain of Baculovirus heliothis expressing an insect-specific toxin of the scorpion Leiurus quinquestriatus hebraeus
- Comparison of in vivo activity of virus from a deleted polyhedrin-gene strain of a single-nucleocapsid baculovirus and a multiple-nucleocapsid baculovirus
- Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses
- The establishment of a Colorado potato beetle (Coleoptera : Chrysomelidae) pupal cell line
- 浮遊培養条件下でのタバコガHelicoverpa zeaクローン細胞系の増殖とバキュロウイルスHzSNPVの複製
- Growth of a clonal cell line of Helicoverpa zea (Lepidoptera:Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV
- Baculovirus heliothisの接種量と摂食時間がHelicoverpa zea(鱗翅目:ヤガ科)初齢, 中齢, および終齢幼虫の死亡率に及ぼす影響
- Effects of viral rate and feeding time on mortality of early-, mid-, and late-stadium larvae of Helicoverpa zea (Lepidoptera:Noctuidae) fed Baculovirus heliothis
- Yields of occlusion bodies from Heliothis virescens(Lepidoptera:Noctuidae)and Helicoverpa(Heliothis) zea(Lepidoptera:Noctuidae)larvae fed wild or recombinant strains of baculoviruses
- Per os and intrahemocoelic activity of exocellular and intracellular virus from a wild-type and deleted polyhedrin-gene strain of the nucleopolyhedrovirus of Autographa californica