Sensitivity of heliothine larvae to wild-type and recombinant strains of a single nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus

スポンサーリンク

概要

• 論文の詳細を見る

• Mid-stadium larvae of Helicoverpa zea were equally susceptible to either the wild-type (Wt) or recombinant (RcHz-Lqh) strain of HzSNPV. There was, however, a significant difference (1.8-fold) in the rate of mortality between the two strains. The LT_<50> for the Wt and Rc strain was 3.4±0.1 days and 1.9±0.2 days, respectively. In contrast, there was a significant difference in both the rate and extent of mortality of mid-stadium larvae of H. virescens fed either the Wt or Rc strain of HzSNPV (LC_<50> : Wt, 11.4±0.6OB/cm^2; Rc, 42.2±8.2OB/cm^2. LT_<50> : Wt, 4.1±0.1 days; Rc, 2.8±0.2 days). An 8-fold increase in the viral rate of the WtHzSNPV was needed (800 OB/mm^2) to match the effectiveness of the RcHzLqh strain at 100 OB/cm^2. Increasing the RcHzLqh rate above 100 OB/cm^2 did not significantly reduce the LT_<50> or larval feeding as measured by frass weight. All viral rates of the Rc strains significantly reduced (ca. 6- to 12-fold) mid-stadium larval feeding over that of the control. Differences between the Rc and Wt strains of HzSNPV, using late-stadium H. zea larvae, showed the same tendency as that using mid-stadium larvae, but the differences were not as great (< 2-fold). Mid-stadium larvae of H. virescens were ca. 3-fold more susceptible to RcAcLqh than to either the WtAc or RcAcAa. Both RcAcLqh and RcAcAa strains killed larvae quicker (1.5- to 1.7-fold) and reduced feeding more (1.6- to 1.9-fold) than the WtAc strain. A combination of RcAcLqh and RcAcAa at 5 OB/cm^2 had no additive effect on mortality of H. virescens larvae and the combination was less effective than either Rc strain at 10 OB/cm^2. Unexpectedly, the known level of susceptibility of semi-permissive larvae of H. zea to WtAcMNPV, as measured by percent mortality, LT_<50> and feeding, was significantly enhanced (ca. 2-fold) when H. zea larvae were fed the RcHzLqh strain. In contrast, expression of the Lqh-toxin by RcHzLqh did not significantly increase the susceptibility of H. subflexa, larvae previously reported as being highly resistant to WtHzSNPV.

• 日本応用動物昆虫学会の論文
• 2001-02-25

著者

• Ignoffo Carlo

  Biological Control Of Insects Research Laboratory U.s.department Of Agriculture Agricultural Researc

• Ignoffo C

  U.s. Dep. Agriculture Agricultural Res. Serv. Mo Usa

• Saathoff S

  U.s. Dep. Agriculture Agricultural Res. Serv. Mo Usa

• Wong James

  E.I.DuPont de Nemours Co., Stine-Haskell Research Center, Agricultural Products

• Mclntosh Arthur

  Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea

• McCutchen William

  E.I.DuPont de Nemours Co., Stine-Haskell Research Center, Agricultural Products

• Grasela James

  Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea

• Goodman Cynthia

  Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea

• Saathoff Stephen

  Biological Control of Insects Research Laboratory, U.S.Department of Agriculture, Agricultural Resea

• Mccutchen Williamf

  E.i.dupont De Nemours Co. Stine-haskell Research Center Agricultural Products

• Wong J

  E.i.dupont De Nemours Co. Stine-haskell Research Center Agricultural Products

• Mcintosh A

  Biological Control Of Insects Research Laboratory Usda Agricultural Research Service

• Goodman C

  Biological Control Of Insects Research Laboratory Usda Agricultural Research Service

• Ignoffo Carlo

  Biological Control Of Insects Research Laboratory Agricultural Research Service U.s. Department Of A

• Grasela J.

  Biological Control Of Insects Research Laboratory Usda Agricultural Research Service

• Saathoff Stephen

  Biological Control Of Insects Research Laboratory U.s.department Of Agriculture Agricultural Researc

• Mcintosh Arthur

  Biological Control Of Insects Research Laboratory Agricultural Research Service U.s. Department Of A

• Wong James

  E.i.dupont De Nemours Co. Stine-haskell Research Center Agricultural Products

• Saathoff Stephen

  Biological Control Of Insects Research Laboratory U.s. Department Of Agriculture Agricultural Resear

• Mccutchen William

  E.i.dupont De Nemours Co. Stine-haskell Research Center Agricultural Products

関連論文

• シングルヌクレオキャプシド(HzSNPV)およびマルチヌクレオキャプシド(AcMNPV)バキュロウイルスの野生株と組換え体に対するタバコガ幼虫の感受性
• Sensitivity of heliothine larvae to wild-type and recombinant strains of a single nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus
• バキュロウイルスの野生株または組換体を接種したHeliothis virescensおよびHelicoverpa(Heliothis)zea幼虫における封入体の産生量
• Mortality and feeding of mid-stadium larvae of Helicoverpa zea and Heliothis virescens fed a wild strain or a recombinant strain of Baculovirus heliothis expressing an insect-specific toxin of the scorpion Leiurus quinquestriatus hebraeus
• Comparison of in vivo activity of virus from a deleted polyhedrin-gene strain of a single-nucleocapsid baculovirus and a multiple-nucleocapsid baculovirus
• The establishment of a Colorado potato beetle (Coleoptera : Chrysomelidae) pupal cell line
• 浮遊培養条件下でのタバコガHelicoverpa zeaクローン細胞系の増殖とバキュロウイルスHzSNPVの複製
• Growth of a clonal cell line of Helicoverpa zea (Lepidoptera:Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV
• Baculovirus heliothisの接種量と摂食時間がHelicoverpa zea(鱗翅目:ヤガ科)初齢, 中齢, および終齢幼虫の死亡率に及ぼす影響
• Effects of viral rate and feeding time on mortality of early-, mid-, and late-stadium larvae of Helicoverpa zea (Lepidoptera:Noctuidae) fed Baculovirus heliothis
• Yields of occlusion bodies from Heliothis virescens(Lepidoptera:Noctuidae)and Helicoverpa(Heliothis) zea(Lepidoptera:Noctuidae)larvae fed wild or recombinant strains of baculoviruses
• Virus Production and Plaque-Forming Ability of Helicoverpa zea (Lepidoptera: Noctuidae) Clonal Cell Lines
• Effect of Larval Extract and Proteinase K on the In vitro Infectivity of Heliothis zea Nonoccluded and Alkali-Liberated Virions

もっと見る 閉じる

スポンサーリンク