IMMUNOFLUORESCENCE AND CELLULAR FRACTION STUDIES ON THE MULTIPLICATION OF INFECTIOUS CANINE HEPATITIS VIRUS

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The process of multiplication of ICH virus in the nucleus and cytoplasm of the infected dog kidney cell cultures were studied by cellular fractionation and immunofluorescence techniques. The infectivity was detected in the nuclear fraction 12 hrs after infection. The virus titer was always higher in the nuclear fraction than in the cytoplasmic fraction throughout the course of infection. CF and HA titers were detected in the cytoplasmic fraction 16 hrs after infection, while in the nuclear fraction both were detected after 24 hrs. The titers of these antigens were always found to be higher in the cytoplasmic fraction than in the nuclear fraction. Applying the fluorescent antibody technique using anti-CF antigen and anti-HA antigen sera, specific fluorescence was detected 9 hrs after infection. The CF antigen first appeared in the nucleus as small granules with less intense fluorescence. In the next stage, the fluorescence was found as a fibrous structure. The HA antigen was found throughout the nucleus as granules with bright fluorescence, and never found as a fibrous structure. When the infectious process advanced, no difference was found between the infected cells stained with anti-CF and -HA antigen sera.
北海道大学の論文