プロテアーゼによる固体粒子・たん白質複合汚れの洗浄に及ぼすCaイオンの影響(第1報) : プロテアーゼ・界面活性剤・ビルダー共存系でのプロテアーゼの安定性に及ぼすCaイオンの影響

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In systems composed protease, ionic surfactant (sodium dodecylbenzene sulfonate, DBS), and builder, effect of Ca2+ on protease activity was studied. Polyvinylalcohol (PVA) as nonionic builder, stylenesulfonate-methacrylate (3:7) copolymer (US) and sodium tripolyphosphate (STP) as ionic builder were used. The results were as follows; 1) For occurrence and stabilization of protease activity, addition of Ca2+ was needed. In protease alone system, the protease activity reached maximum at about 10mgL-1. 2) Nonionic builder PVA contributed to the stabilization of protease activity, but DBS and ionic builder, US and STP inhibited it. With increasing Ca2+ concentration in solution, inhibition of protease activity by DBS, US, and STP was dissolved, or reduced. 3) From relationship protease activity and Ca2+ concentration, inhibition by DBS and US were related to those chelation ability for Ca2+. Maintaining Ca2+ concentration at about 10mgL-1 as final Ca2+ concentration produced high activity of protease as in protease alone system. In system containing US, Ca2+ concentration as final Ca2+ concentration was over 50mgL-1. 4) Inhibition by STP was related not only chelation for Ca2+ but also obstruction to form three-dimensional structure of protease for occurrence of protease activity.
松山東雲女子大学・松山東雲短期大学の論文