〈原著〉エタノール処理によりHepG2細胞の培養上清に出現するコラーゲン産生促進因子の解析

概要

論文の詳細を見る
Alcohol-induced hepatic fibrogenesis occurs in the absence of any obvious hepatocellular inflammation or tissue necrosis. However, the precise mechanism of this fibrosis has not been fully elucidated. We previously found that collagen stimulating activity was detected in the conditioned medium of ethanol-exposed human hepatoblastoma HepG2 cells. The aim of this study is to characterize this factor which facilitates hepatic fibrosis in alcoholic liver disease. Type I procollagen peptide (PIC) synthesis by human fibroblast IMR-90 markedly increased after incubation with the conditioned medium of ethanol-treated HepG2 cells. The collagen stimulating activity was immuno-absorbed with anti-transforming growth factor (TGF)-α antibodies in a dosedependent manner. TGF-α in the conditioned medium of ethanol-treated HepG2 and serum TGF-α levels in patients with alcoholic liver disease were determined using an enzyme-linked immunosorbent assay. TGF-α in the conditioned medium of ethanol-treated HepG2 was significantly increased. Serum TGF-α levels in patients with liver fibrosis in alcoholic liver disease were significantly higher than those in chronic viral hepatitis and viral cirrhosis. In conclusion, TGF-α is one of the causative factors for the development of hepatic fibrosis in alcoholic liver disease.
1997-08-01