Production of Type 2 Dengue (D2) Monoclonal Antibody and Cell Culture Derived D2 Antigen for Use in Dengue IgM-Capture ELISA
スポンサーリンク
概要
- 論文の詳細を見る
In order to strengthen laboratory diagnostic capability on dengue by IgM-ELISA in Malaysia, type 2 dengue (D2) monoclonal antibody (MAb) and cell culture-derived D2 antigen were prepared as culture fluid of hybridoma cell line and D2-infected C6/36 culture fluid, respectively. Comparative IgM-ELISA was carried out on 90 serum specimens from dengue patients using newly prepared reagents in comparison with routine reagents (D2-MAb mouse ascitic fluid from CDC, USA, and D2 antigen extracted from infected suckling mouse brains). The newly prepared MAb at 2-10 fold higher concentration than that estimated by antigen detection ELISA, in combination with the undiluted D2-infected C6/36 cell culture fluid, provided comparable sensitivity (93.3-97.9%) to that of routine reagents (90.0-98.5%).
- 長崎大学の論文
著者
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Igarashi Akira
Department Of Applied Chemistry Faculty Of Engineering Kogakuin University
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Mohamed Haliman
Division Of Virology Institute For Medical Research
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Yusof Apandi
IMR-JICA Project,I-nstitute for Medical Reserch
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