Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas

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概要

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• This study was undertaken to determine whether expressions of the vascular endothelial growth factor C (VEGF-C) and D (VEGF-D) correlate with clinicopathological parameters, with particular reference to lymph node metastasis in gastric carcinoma. Based on the data, as a preliminary study, we investigated whether VEGFC expression could be a predictor of lymph node metastasis. Twenty-two surgical specimens of gastric carcinomas with (n=14) or without (n=8) lymph node metastasis were studied. The mRNA levels of VEGF-C and VEGF-D were quantified using real-time quantitative (RTQ) RT-PCR, a new method of kinetic quantitative PCR. We then calculated the tumor:normal VEGF-C and VEGF-D mRNA level ratios for each specimen which were designated as the normalized mRNA value. The normalized mRNA values of VEGF-C in primary tumors correlated well with lymph node metastasis but that of VEGF-D did not. We then arbitrarily chose the cut-off point for the normalized mRNA values of VEGF-C based on the mRNA values of patients without lymph node metastasis (mean + SD); we examined the data to see if the values above the cut-off point would indicate the presence of lymph node metastasis. The sensitivity and specificity of the normalized mRNA values of VEGF-C for the diagnosis of lymph node metastasis were 85.2% and 75.0%, respectively. These results suggest that the VEGF-C may play an important role in lymph node metastasis in gastric carcinoma and that VEGF-C may be a useful diagnostic marker for lymph node metastasis.

• 札幌医科大学の論文

著者

• HIRATA KOICHI

  First Department of Surgery, Sapporo Medical University School of Medicine

• KIMURA YASUTOSHI

  First Department of Surgery, Sapporo Medical University School of Medicine

• YAMAGUCHI KOJI

  First Department of Surgery Kyushu University Faculty of Medicine

• SASAKI KAZUAKI

  Department of Surgery, Doto Hospital, A Not-For-Profit Medical Foundation

• Mukaiya Mitsuhiro

  First Department of Surgery,Sapporo Medical University School of Medicine

• HATA FUMITAKE

  First Department of Surgery, Sapporo Medical University School of Medicine

• Hirata K

  札幌医科大学 生化学

• Hata Fumitake

  札幌医科大学 医学部外科学第一講座

• Hata F

  Department Of Surgery Sapporo Medical University School Of Medicine

• Hata Fumitake

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Hata Fumitake

  Department Of Surgery Sapporo Medical University School Of Medicine

• Hata Fumitake

  札幌医科大学 第1外科

• Hata Fumitake

  札幌医科大学 解剖

• Hata Fumitake

  First Department Of Surgery Sapporo Medical University

• Hirata K

  Department Of Surgery Sapporo Medical University School Of Medicine

• Hirata K

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Sasaki K

  Department Of Surgery Sapporo Medical University School Of Medicine

• Yamaguchi Koji

  First Department Of Surgery Sappro Medical University School Of Medicine

• FURUHATA TOMOHISA

  First Department of Surgery, Sapporo Medical University, School of Medicine

• KATSURAMAKI TADASHI

  First Department of Surgery, Sapporo Medical University, School of Medicine

• Kawakami Masayo

  First Department of Surgery, Sapporo Medical University School of Medicine

• Kihara Chikashi

  First Department of Surgery, Sapporo Medical University School of Medicine

• Okita Kenji

  First Department of Surgery, Sapporo Medical University School of Medicine

• Hata Fumitake

  Department Of Surgery (section 1). Sapporo Medical University School Of Medicine

• OKITA Kenji

  Sapporo Medical University, First Department of Surgery

• Sasaki Kazuaki

  Department Of Anatomy And Anthropology Tohoku University School Of Medicine

• Hirata Koichi

  First Department Of Surgery Sapporo Medical University

• Kimura Y

  Sapporo Medical University First Department Of Surgery

• Kimura Yasutoshi

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Okita Kenji

  Sapporo Medical University First Department Of Surgery

• Katsuramaki T

  Department Of Surgery I Sapporo Medical University School Of Medicine

• Kawakami Masayo

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Kawamoto Masaki

  Department Of Surgery I Sapporo Medical University Hospital

• Mukaiya M

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Mukaiya Mitsuhiro

  First Department Of Surgery Sapporo Medical University

• Kojima T

  Department Of Innovative And Engineered Materials Interdisciplinary Graduate School Of Science And E

• Furuhata T

  Sapporo Medical University First Department Of Surgery

• Kihara Chikashi

  First Department Of Surgery Sapporo Medical University School Of Medicine

• Kojima Takashi

  米国

• Sogahata Katsuya

  Department Of Surgery Sapporo Medical University School Of Medicine

• Hirata Koichi

  First Department Of Surgery Cancer Research Institute Sapporo Medical University

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