Down-regulation of c-myc protein expression in g0/g1 phase of the cell cycle during the induction of differentiation by 1,25 dihydroxyvitamin D3 in HL-60 cells

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概要

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• It is well established that c-myc expression is down-regulated during induction of differentiation in various leukemia cell lines including HL-60 human myeloid leukemia cells. Previously we have shown that c-myc protein expression was specifically down-regulated in G0/G1 phase of the cell cycle during induction of granulocytic differentiation with dimetylsulfoxide (DMSO) in HL-60 cells. In the present study, we examined whether c-myc protein expression is also down-regulated in G0/G1 phase during monocytic differentiation of HL-60 cells. HL-60 cells can be induced to differentiate along monocytic lineage by treatment with 1,25 dihydroxyvitamin D3 (D3). C-myc protein expression was down-regulated after 72h of treatment with D3 in HL-60 cells. Simultaneous analysis of protein level and cell cycle using a two-color flow cytometry showed that the down-regulation of c-myc protein expression occurred specifically in G0/G1 phase. Down-regulation of c-myc protein expression in G0/G1 phase was more clearly shown by using synchronized cell population that was arrested at G0/G1 phase by serum starvation. Furthermore, G0/G1-synchronized cells showed a higher susceptibility to differentiation in response to D3. These results suggest that c-myc protein is down-regulated in G0/G1-specific phase during not only granulocytic differentiation but also monocytic differentiation, and down-regulation of c-myc protein expression in G0/G1 phase is involved in the program of differentiation of HL-60 cells.

• 島根大学の論文

著者

• Kobayashi Shotai

  島根大学 医学部神経内科

• ISHIKURA Hiroto

  Division of Clinical Study of Oncology, Shimane University Hospital

• Kobayashi Shotai

  Department Of Neurology Hematology & Rheumatology School Of Medicine Shimane University

• Kobayashi S

  Shimane Medical Univ. Izumo Jpn

• Kobayashi Shotai

  Third Department Of Internal Medicine Shimane University School Of Medicine

• Sato Y

  Fukushima Medical Univ. School Of Medicine

• KUMAKURA Shunichi

  Division of Blood Transfusion, School of Medicine, Shimane University

• ISHIKURA Hiroto

  Department of Clinical Nursing, Shimane University

• Kumakura Shunichi

  Third Division of Internal Medicine, Shimane Medical University

• Tsumura Hiroto

  Third Division of Internal Medicine, Shimane Medical University

• Nakashima Akemi

  Third Division of Internal Medicine, Shimane Medical University

• Sato Yukie

  Third Division of Internal Medicine, Shimane Medical University

• Sato Yukie

  Third Division Of Internal Medicine Shimane Medical University

• Kobayashi Shotai

  Third Department Of Internal Medicine Shimane Medical University

• Tsumura Hiroto

  Third Division Of Internal Medicine Shimane Medical University

• Ishikura Hiroto

  Department Of Clinical Nursing Shimane University

• Ishikura Hiroto

  Division Of Clinical Study Of Oncology Shimane University Hospital

• Kumakura Shunichi

  Division Of Blood Transfusion School Of Medicine Shimane University

• Nakashima Akemi

  Third Division Of Internal Medicine Shimane Medical University

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