<Originals>Correlation between immunohistochemical reaction with anti-actin antibody and contractility in ischemic hearts of rats

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An attempt was made to clarify the correlation between the loss of reaction with anti-actin antibody and the cardiac contractility in ischemia. After 5 minutes of ischemia followed by 30 minutes of reperfusion, the complete disappearance of anti-actin antibody reaction was observed in the subendocardial region, and cardiac contractility had significantly decreased to 95.4%. After 30 minutes of ischemia followed by 30 minutes of reperfusion, Evans blue began to leak into the ischemic area, which invariably indicating the occurrence of edema. In hematoxylin and eosin (HE) staining, eosinophilic degeneration was shown in the ischemic region and the anti-actin antibody reaction in muscle fibers could no longer be detected in any layer of the ventricular wall. Contractility had significantly decreased to 22.1%. After 6 hours of ischemia, the triphenyltetrazolium chloride (TTC) reaction ceased to occur in the ischemic region, almost all the fibers showed severely eosinophilic degeneration or necrosis, and the anti-actin antibody reaction no longer took place. The disappearance of the anti-actin antibody reaction thus appears to be correlated to the decrease or the loss of cardiac contractility.
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