<Originals>Detection of early ischemic myocardial damage using immunohistochemical technique with anti-actin antibody in humans and rats
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The effectiveness of diagnosing myocardial infarction, and changes of actin filaments were investigated in the human autopsy cases and the experimental ischemia of rats, by using immunohistochemical methods with anti-actin antibodies produced from chicken gizzard. In acute myocardial infarction of human materials, the diminished areas of immunohistochemical reactions with anti-actin antibodies were slightly larger than the areas losing the triphenyltetrazolium chloride (TTC) reaction. In the experimental study, the left coronary arteries of rats were ligated to produce ischemia. In the cases of ischemia lasting for 5,15 and 30 minutes, TTC reaction was shown, but the anti-actin antibody reaction disappeared in the areas corresponding to the ischemic sites. While actin filaments were clearly revealed on electron microscopic (EM) examination of these sites. In the case of ischemia lasting for more than 6 hours, the anti-actin antibody reaction had disappeared, corresponding to the disappearance of the TTC reaction. At this stage, actin filaments were revealed by EM examination, These results indicate that ischemia lasting more than 5 minutes induces some type of biochemical degeneration at the molecular level of myocardial actin, most likely the change of actin polymerization. Moreover, they show that the anti-actin antibody technique is capable of detecting such very early degenerative and ischemic changes, proving itself to he better suited for early determination of the range and degree of ischemia in humans and rats.
- 近畿大学の論文
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