Loss of poly-γ-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene
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概要
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The mobile genetic element IS4Bsu1 of Bacillus subtilis (natto), the commercial strain used for natto fermentation, was found to spontaneously translocate to the swrA gene, which led to a defect in poly-γ-glutamic acid (γPGA) synthesis. The γPGA defect of NAFIS1 (swrA::IS4Bsu1) was restored by a plasmid harboring the swrA gene. The insertion of IS4Bsu1 to the swrA gene appeared not to interfere with minJ gene expression, located downstream of the swrA gene, because a defect in cell motility exhibited by NAF16 (minJ::Spcr) was not observed in NAFIS1 (swrA::IS4Bsu1). We found that the minJ gene is essential for γPGA synthesis.
- 2011-09-01
著者
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Tran Lam-son
Division Of Applied Microbiology National Food Research Institute
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Kimura Keitarou
Div. Of Applied Microbiology National Food Res. Inst.
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Kimura Keitarou
Division Of Applied Microbiology National Food Research Institute
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Funane Kazumi
Division Of Applied Microbiology National Food Research Institute
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