Effects of Hydrostatic High Pressure on the Structure and Antibacterial Activity of Recombinant Human Lactoferrin from Transgenic Rice

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概要

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• High pressure was applied to recombinant human lactoferrin obtained from rice (rhLF) and its effect was evaluated on the structure and activity of the protein. Treatments of 400, 500, and 650 MPa for 15 min (20 °C), were applied to rhLF at 2 mg/mL in three iron-saturation forms. The structural characteristics of the treated proteins were analyzed by differential scanning calorimetry (DSC) and by fluorometric analysis, and immunoreactivity by ELISA. Iron retention and binding properties and antibacterial activity against <I>Escherichia coli</I> O157:H7 were also studied. The results obtained indicate that the treatments at 400 and 500 MPa did not greatly modifiy the conformation of lactoferrin, meanwhile treatment at 650 MPa affected in different degrees the three forms of rhLF. With respect to antibacterial activity, only apo rhLF showed antibacterial activity against <I>E. coli</I>, activity that was maintained after treatment at 400 MPa, while holo and AsIs rhLF did not inhibit the growth of <I>E. coli</I>.

• 2012-01-23

著者

• CALVO Miguel

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• Calvo Miguel

  Food Technology And Biochemistry Department Faculty Of Veterinary Science University Of Zaragoza

• Sanchez Lourdes

  Dep. Of Animal Production And Food Sci. Food Technol. Unit Veterinary Fac. Univ. Of Zaragoza

• Perez Maria-dolores

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Sanchez Lourdes

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Castillo Eduardo

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Franco Indira

  Department Of Food Technology Veterinary Faculty University Of Zaragoza

関連論文

• Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers
• Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment
• Interaction of Mercury with Human and Bovine Milk Proteins
• Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers
• Antibacterial Activity of Recombinant Human Lactoferrin from Rice : Effect of Heat Treatment
• Effects of Hydrostatic High Pressure on the Structure and Antibacterial Activity of Recombinant Human Lactoferrin from Transgenic Rice

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