Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers

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概要

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• The possibility of using recombinant human lactoferrin from rice (rhLF) makes it necessary to study its differences from the protein of milk. In this work, the binding of different iron-saturated forms of rhLF to Caco-2 cells was studied. Iron-saturated rhLF bound in higher proportion than the apo-form, but, the data obtained for specific binding were not compatible with receptor-mediated binding. Competition assays showed the same binding capacity for human milk lactoferrin as for rhLF to Caco-2 cells. Another basic protein of milk, lactoperoxidase, was found to compete with rhLF for binding to Caco-2 cell membranes, suggesting an electrostatic interaction. The transport of iron (59Fe) bound to rhLF and to citrate and the transport of rhLF (125I-labeled) were studied on Caco-2 monolayers. Transport of iron was found to be significantly greater when bound to citrate than to rhLF. The amount of intact lactoferrin that traversed the Caco-2 monolayers was very low, suggesting degradation of it across these cells.

• 社団法人 日本農芸化学会の論文
• 2009-12-23

著者

• Conesa Celia

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• Pocovi Coloma

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• Perez Maria-Dolores

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• CALVO Miguel

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• SÁNCHEZ Lourdes

  Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, Universi

• Calvo Miguel

  Food Technology And Biochemistry Department Faculty Of Veterinary Science University Of Zaragoza

• Sanchez Lourdes

  Dep. Of Animal Production And Food Sci. Food Technol. Unit Veterinary Fac. Univ. Of Zaragoza

• Perez Maria-dolores

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Sanchez Lourdes

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Conesa Celia

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

• Pocovi Coloma

  Department Of Animal Production And Food Science Food Technology Unit Veterinary Faculty University

関連論文

• Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers
• Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment
• Interaction of Mercury with Human and Bovine Milk Proteins
• Transport of Iron Bound to Recombinant Human Lactoferrin from Rice and Iron Citrate Across Caco-2 Cell Monolayers
• Antibacterial Activity of Recombinant Human Lactoferrin from Rice : Effect of Heat Treatment
• Effects of Hydrostatic High Pressure on the Structure and Antibacterial Activity of Recombinant Human Lactoferrin from Transgenic Rice

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