Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection
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概要
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The objective of this study was to determine a possible application of hairy roots as biotechnological producers of furanocoumarins, by establishing root clones of Glehnia littoralis after infection from Agrobacterium rhizogenes. None of the root clones grew under hormone-free conditions, but when cultured in the presence of indole-3-butyric acid, growth varied substantially across clones. Analysis of the insertion and expression of pathogenic rol (A, BTL, BTR, and C) genes for 10 clones showed that the stable expression of rolC and irregular expression of rolB were detected in 2 clones, which exhibited active growth and higher furanocoumarin induction. Following ascorbic acid treatment, the productivity of xanthotoxin and bergapten in a root clone harbouring a pathogenic gene was 2.8 and 3.7 times greater than that recorded in wild-type roots, respectively. These results indicate that root cultures possessing pathogenic rol genes offer a potential means to produce furanocoumarins.
- 2011-06-25
著者
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KITAMURA Yoshie
Faculty of Environmental Studies, Nagasaki University
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Ishikawa Aya
Graduate School Of Biomedical Sciences Nagasaki University
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Ozeki Yoshihiro
Fac. Of Engineering Tokyo Univ. Of Agriculture And Technol.
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Kitamura Yoshie
Fac. Of Environmental Studies Nagasaki Univ.
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Kitamura Yoshie
Faculty Of Environmental Studies Nagasaki University
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Terato Masahiro
Graduate School of Biomedical Sciences, Nagasaki University
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Ozeki Yoshihiro
Faculty Of Engineering Tokyo University Of Agriculture And Technology
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Yamada Koji
Graduate School Of Biomedical Sciences Nagasaki University
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Terato Masahiro
Graduate School Of Biomedical Sciences Nagasaki University
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