ラット肩関節不動化モデルにおける表層軟骨細胞層の変化

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Superficial Zone Protein (SZP)/ Lubricin is expressed by superficial zone chondrocytes, synoviocytes, and tenocytes. SZP is essential for boundary lubrication of joints. In some previous reports, SZP expression is up-regulated by shear stress. Stimulation by TGF- β1 also up-regulates SZP expression. We hypothesized that joint immobilization might induce the decrease of SZP expression, since shear stress might decrease. The aim is to investigate the number of superficial zone chondrocytes and mRNA expression in chondrocytes in humerus head; SZP, TGF- β1, type II collagen, and aggrecan. Extra-articular shoulder immobilization was performed in Sprague-Dawley 12-week-old male rats. To evaluate the cell number, histologic sections were made after 8-week immobilization (N = 6). The number of chondrocytes existing within 20μm depth was counted both in the contact area and non-contact area. To evaluate mRNA expression, chondrocytes were isolated from whole cartilage of the humeral head after 4-week immobilization. RNA was isolated and Quantitative real-time PCR assay was carried out (N = 8). In the statistical analysis, Student t-test was performed. In the immobilized group, the number of superficial zone chondrocytes decreased significantly only in the contact area. SZP and TGF- β1 mRNA expression decreased significantly in the immobilized group. Aggrecan expression increased significantly in the immobilized group. There was no significant difference between the two groups in type II collagen expression. In the current study, SZP expression was decreased in immobilized joints. We considered that immobilization reduced the SZP expression and a decrease in the number of chondrocytes. It was suggested that loss of shear stress induced this results. Decrease of TGF- β1 expression might be related to the decrease in SZP expression.
2010-08-04

ラット肩関節不動化モデルにおける表層軟骨細胞層の変化

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