Sanguinarine as a Potent and Specific Inhibitor of Protein Phosphatase 2C in Vitro and Induces Apoptosis via Phosphorylation of p38 in HL60 Cells
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概要
- 論文の詳細を見る
Sanguinarine, a plant alkaloid, was identified as a potent and specific protein phosphatase (PP) 2C inhibitor. It inhibited PP2C competitively with respect to α-casein (Ki=0.68 μM) and showed selectivity for PP2C as compared with PP1, PP2A, and PP2B in vitro. In vivo, sanguinarine showed cytotoxicity toward human promyelocytic leukemia cell line HL60, with an IC50 value of 0.37 μM, and induced apoptosis through a caspase-3/7-dependent mechanism involving the phosphorylation of p38, a PP2Cα substrate. The apoptosis activity induced by sanguinarine was partially inhibited by a p38 inhibitor, SB203580, and was involved in the phospho-p38 protein in HL60 cells.
- 社団法人 日本農芸化学会の論文
- 2010-03-23
著者
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ABURAI Nobuhiro
Laboratory of Chemical Biology, Faculty of Agriculture, Iwate University
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Ohnishi Motoko
Graduate School Of Bioscience And Biotechnology Chubu Univ.
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Ohnishi Motoko
Graduate School Of Bioscience And Biotechnology Chubu University
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Kimura Ken-ichi
Laboratory Of Biology Sapporo Campus Hokkaido University Of Education
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Kimura Ken-ichi
Laboratory Of Chemical Biology The United Graduate School Of Agricultural Sciences Iwate University
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YOSHIDA Mami
Graduate School of Bioscience and Biotechnology, Chubu University
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Aburai Nobuhiro
Laboratory Of Chemical Biology The United Graduate School Of Agricultural Sciences Iwate University
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Yoshida Mami
Graduate School Of Bioscience And Biotechnology Chubu University
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Aburai Nobuhiro
Laboratory Of Chemical Biology Faculty Of Agriculture Iwate University
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KIMURA Ken-ichi
Laboratory of Biochemistry, Deparment of Agricultural Chemistry, Tohoku University
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