E275 and F276 in β12-β13 Loop of Protein Phosphatase-1 Resist Mn^<2+>-Mediated Activation
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概要
- 論文の詳細を見る
Protein phosphatase-1 (PP1) is one of the most important Ser/Thr phosphatases in eukaryotic cells. G274, E275, and F276 are located at the tip of the β12-β13 loop of protein phosphatase-1. Without Mn2+, the basal activities and intrinsic fluorescence spectra of all single and double deletion mutants of G274, E275, and F276 were similar to those of PP1, but deletion mutants ΔE275 and ΔE275F276 showed hyperactivity and corresponding changes in intrinsic fluorescence spectra when Mn2+ was present. This suggests that the conformation transition resulting from the combined effect of mutation and Mn2+ accounts for the hyperactivity of mutants. These observations imply that E275 and F276 play a role in resisting enzyme activation by Mn2+ in PP1.
- 社団法人 日本農芸化学会の論文
- 2009-04-23
著者
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Wei Qun
Department Of Biochemistry And Molecular Biology Beijing Normal University Beijing Key Laboratory
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Huang Wei
Department Of Biochemistry And Molecular Biology Beijing Normal University Beijing Key Laboratory
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XIE Xiujie
Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory
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XUE Chengzhe
Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory
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Xie Xiujie
Department Of Biochemistry And Molecular Biology Beijing Normal University Beijing Key Laboratory
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Xue Chengzhe
Department Of Biochemistry And Molecular Biology Beijing Normal University Beijing Key Laboratory
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HUANG Wei
Department of Aeronautics and Astronautics, National Cheng Kung University
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