Molecular Cloning of Functional Genes for High Growth-Temperature and Salt Tolerance of the Basidiomycete Fomitopsis pinicola Isolated in a Mangrove Forest in Micronesia
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概要
- 論文の詳細を見る
Several functional genes encoding putative proteins, heat shock protein 70, sphingosine phosphate lyase, and Na+/H+ antiporter, were cloned from the basidiomycete Fomitopsis pinicola, a wood-rotting fungus isolated in the tropical mangrove forest of Pohnpei Island of the Federated States of Micronesia. The deduced amino acid sequences of the obtained genes involved in heat shock resistance, lipid synthesis, and salt tolerance showed diverse similarities to other homologous proteins. Molecular phylogenetic trees of these proteins suggested that encoded proteins of the cloned genes of F. pinicola differed remarkably from other homologs in various organisms, even fungal proteins. Putative candidates for other genes related to several cellular metabolisms were also amplified, implying the possible existence of those genes in F. pinicola. This is the first report of possibly functional genes derived from a basidiomycetous mushroom growing in tropical islands such as Micronesia. The genes found in this study might play important roles in the cellular survival of the basidiomycete F. pinicola under severe environmental conditions.
- 社団法人 日本農芸化学会の論文
- 2007-01-23
著者
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Shibuya Hajime
Institute Of Applied Biochemistry University Of Tsukuba
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澁谷 源
森林総研
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MIYAZAKI Yasumasa
Department of Applied Microbiology and Mushroom Sciences, Forestry & Forest Products Research Instit
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Shibuya Hajime
Department Of Applied Microbiology Forestry And Forest Products Research Institute
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HIRAIDE Masakazu
Department of Applied Microbiology, Forestry and Forest Products Research Institute
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Miyazaki Yasumasa
Forestry And Forest Products Res. Inst.
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Miyazaki Yasumasa
Department Of Applied Microbiology And Mushroom Sciences Forestry & Forest Products Research Ins
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Hiraide Masakazu
Department Of Applied Microbiology Forestry And Forest Products Research Institute
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