Transformation of the Mycorrhizal Basidiomycetes, Suillus grevillei and S. bovinus, by Particle Bombardment
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概要
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Mycorrhizal basidiomycetes, Suillus grevillei and S. bovinus, were transformed by particle bombardment. We isolated eight and four transformants from S. grevillei and S. bovinus respectively under three conditions differing in terms of target distance and helium pressure. The transformation frequencies were 1.2 and 0.6 transformants/μg DNA. Plasmid DNAs were integrated into the genomic DNA of the transformants and stably maintained. This is the first description of the transformation of S. grevillei and S. bovinus by particle bombardment.
- 社団法人 日本農芸化学会の論文
著者
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MURATA Hitoshi
Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Scien
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NAKAMURA Masaya
Department of Applied Microbiology, Forestry and Forest Products Research Institute
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Sunagawa Masahide
Department Of Applied Microbiology Forestry And Forest Products Research Institute
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MIYAZAKI Yasumasa
Department of Applied Microbiology and Mushroom Sciences, Forestry & Forest Products Research Instit
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