Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos

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概要

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• The aims of the present study were twofold. The first aim was to examine whether or not early pregnancy-associated thrombocytopenia (EPAT) occurred in cows following nonsurgical embryo transfer by observing the daily patterns of the concentration of peripheral platelets (PLT) during the preimplantation period of pregnancy. The other goal was to assess the use of PLT count as a diagnosis of early pregnancy in cows. In Experiment 1, out of 50 Holstein parous cows, 40 had one embryo nonsurgically transferred to each uterine horn on Day 6 or 7 (Day 0=onset of standing estrus). The remaining 10 cows served as controls having no embryos transferred. Blood was taken daily at the same time, from Day 6 or 7 following estrus (the day when the embryos were transferred in the non-controls) to the day of return to estrus for control cows and non-pregnant cows, or to Day 36 for pregnant cows. Bilateral embryo transfers resulted in 9 twin, 14 single, and 17 non-pregnancies. Some recipient cows showed an increase in PLT following embryo transfer, while others displayed a decrease in PLT. If significant PLT change was characterized either by a decrease or an increase of >20% compared to the value of pre-transfer, the proportions of cows indicating significant change were 78% (7/9) in twin, 64% (9/14) in single, 76% (13/17) in non-pregnancies and 30% (3/10) in controls. The proportions in twin (P<0.05), single (P<0.05) and non-pregnancies (P<0.01) were higher than that in controls. The effect of the particular group on PLT was significant (P<0.0025). In Experiment 2, each of several in vitro-produced blastocysts were nosurgically transferred to 9 Japanese Black cows on Day 7 or 8 following onset of estrus. Seven days after embryo transfer, an attempt to recover the previously transferred embryos by nonsurgical flushing was performed. Embryos could be recovered on Day 14 or 15 from 5 animals, whose blood was taken from the day of embryo transfer to the next day of embryo recovery and during the corresponding days of the following estrous cycle. For PLT, differences were detected between the both phases of 3 out of the 5 cows. The individual PLT variation in response means that EPAT does not necessarily occur in cows following embryo transfer, and that the use of PLT counts is unlikely to be clinically useful in assessing early pregnancy in cows. These results may suggest, however, that maternal platelet activation would occur during early pregnancy in cattle.

• 日本繁殖生物学会の論文
• 1996-11-01

著者

• KOJIMA Toshiyuki

  National Livestock Breeding Center

• SHIMIZU Manabu

  Department of Electrical Engineering, Osaka University

• Shimizu M

  National Agricultural Res. Center For Tohoku Region Iwate

• ZENIYA Yumie

  Department of Animal Reproduction, National Institute of Animal Industry, Tsukuba-Norindanchi POBox

• SHIMIZU Manabu

  National Agricultural Research Center for Tohoku Region

• TOMIZUKA Tsuneo

  Department of Animal Reproduction, National Institute of Animal Industry

• Kojima Toshiyuki

  National Livestock Breeding Center Ministry Of Agriculture Forestry And Fisheries

• AKAGI Satoshi

  Kyushu National Agricultural Experiment Station Nishigoshi-machi

• Kojima T

  National Livestock Breeding Center Fukushima Jpn

• Zeniya Yumie

  Department Of Animal Reproduction National Institute Of Animal Industry

• Tomizuka Tsuneo

  Department Of Animal Reproduction National Institute Of Animal Industry

• Shimizu Manabu

  Department Of Chemistry For Materials Faculty Of Engineering Mie University

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