Effect of Dietary Administration with Linoleic Acid and α-Tocopherol on Freezing Tolerance in Porcine Embryos

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概要

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• The study on cryopreservation of porcine embryos were conducted to determine an effect of dietary intake of polyunsaturated fatty acid by donor gilts on embryo survival after freezing and thawing. Linoleic acid and a-tocopherol were supplemented to a conventional concentrated diet at the rate of 3.75% and 600 ppm, respectively. The diet was given to 5 pubertal gilts with an initial age of 6 months old for approximately 4 months (2 to 3 kg/head/day). From at least 70 days after starting of feeding of the experimental diet, embryo collections were performed. The control embryos were obtained from pubertal gilts given only the conventional concentrated diet. Embryos were surgically collected by flushing both uteri of donors on day 6 (day 1=the last day of estrus). Expanded blastocysts were used immediately for experiment, after measuring the diameter by an ocular micrometer. The younger-stage embryos were cultured in vitro in Hams F-12 supplemented with 12 mg/ml BSA fraction V in 5% CO2 in air at 38.5 C up to expanded blastocyst stage with more than 200 μm in diameter. The embryos were equilibrated with 10% ethylene glycol solution containing 0.2 M trehalose for 5 min at room temperature. The embryos loaded into 0.25-ml plastic straws were immersed in alcohol bath at -7 C, seeded at -7 C, cooled to -30 C at the rate of 0.4 C/min and plunged directly into liquid nitrogen. After being thawed in air, the embryos were subjected to count the viable nuclei following a two-step dilution of the cryoprotectant with 0.5 M trehalose solution. As a result of counting the viable nuclei of frozen-thawed embryos, the mean number of viable nuclei of the embryos derived from gilts given the linoleic-acid supplemented diet was larger (P=0.0553) than that of the embryos derived from gilts given the control diet. The result of the present study suggests that dietary linoleic acid intake by embryo donor gilts is likely to have a potential effect on enhancement of freezing tolerance in porcine embryos.

• 日本繁殖生物学会の論文
• 1996-02-01

著者

• KOJIMA Toshiyuki

  National Livestock Breeding Center

• Kojima Toshiyuki

  National Livestock Breeding Center Ministry Of Agriculture Forestry And Fisheries

• YOSHINO Junryo

  Miyagi Prefectural Animal Industry Experiment Station

• Kojima T

  National Livestock Breeding Center Fukushima Jpn

• Zeniya Y

  Department Of Animal Reproduction National Institute Of Animal Industry

• Zeniya Yumie

  Department Of Animal Reproduction National Institute Of Animal Industry

• Totsukawa Kiyoshi

  Faculty Of Agriculture Yamagata University

• IWASAKI Taizo

  Faculty of Agriculture, Yamagata University

• Iwasaki Taizo

  Faculty Of Agriculture Yamagata University

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• Birth of Cloned Calves Derived from Cultured Oviductal Epithelial Cells of a Dairy Cow
• The Birth of Piglets from Embryos Cultured for 3 to 5 Days in a Simple Salt Solution Lacking Glucose
• Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos
• Effect of Administration with Low-dose FSH to Recipient Cows on Embryonic Survival after Bilateral Nonsurgical Embryo Transfer
• Dietary Administration of Fatty Acids-Enriched Mold Dried Cell Containing γ-Linolenic Acid to Female Pigs Improves Ovulation Rate and Embryo Quality in Summer
• Dietary Administration of Fatty Acids-Enriched Mold Dried Cell Containing γ-Linolenic Acid to Female Pigs Improves Ovulation Rate and Embryo Quality in Summer
• Occurrence of Early Pregnancy-Associated Thrombocytopenia in Splenectomized Rabbits
• Effect of Dietary Administration with Linoleic Acid and α-Tocopherol on Freezing Tolerance in Porcine Embryos
• Cloned Cows with Short Telomeres Deliver Healthy Offspring with Normal-length Telomeres
• Farrowing of Normal Piglets by Transfer of Biopsied Porcine 4-cell Embryos Following In Vitro Culture

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